Luria Bertani HiCynth™ Broth, Miller (Miller Luria Bertani HiCynth™ Broth) is recommended for the cultivation and maintenance of recombinant strains of Escherichia coli for genetic and molecular biology studies; may be used for routine cultivation and estimation of not particularly fastidious microorganisms. Luria Bertani HiCynth™ Broth,Miller is used for cultivation and maintenance of recombinant strains of Escherichia coli. The media is nutritionally rich for the growth of pure cultures of recombinant strains. Strains derived from Escherichia coli K12 are deficient in Vitamin B synthesis which are further modified by specific mutation to create auxotrophic strains and are therefore unable to grow on nutritionally deficient media. This medium is prepared by replacing animal and vegetable peptones with chemically defined peptones to avoid BSE/TSE risks associated with animal peptones.