Luria Bertani HiVeg™ Agar, Miller (Miller Luria Bertani HiVeg™ Agar) is recommended for the cultivation and maintenance of recombinant strains of Escherichia coli for genetic and molecular biology studies. It may be used for routine cultivation and estimation of not particularly fastidious microorganisms. Hiveg media is nutritionally rich for the growth of pure cultures of recombinant strains of Escherichia coli for genetic and molecular studies and may be used for routine cultivation of not particularly fastidious microorganisms. Strains derived from Escherichia coli K12 are deficient in Vitamin B synthesis are further modified by specific mutation to create auxotrophic strains and are therefore unable to grow on nutritionally deficient media. This medium is prepared by replacing animal peptones with vegetable defined peptones to avoid BSE/TSE/GMO risk.