This animal free medium is used for cultivation of yeasts, moulds and aciduric microorganisms, recommended by APHA. The acid reaction of the final medium is inhibitory to a large number of bacteria making it particularly useful for cultivating fungi and aciduric microorganisms. For isolation of fungi from
contaminated specimens, a selective medium should be inoculated simultaneously. Incubate cultures for 4 to 6 weeks before reporting as negative.