Description
Perfringens HiVeg Agar Base (O.P.S.P.) with selective supplements is used as a selective medium for isolation and enumeration of Clostridium perfringens in food. Perfringens HiVeg Agar with supplements gives high degree of selectivity and specificity.The antibiotics sulphadiazine, oleandomycin and polymyxin B make the medium highly selective inhibiting sulphite-reducing bacteria other than C. perfringens such as Salmonella, Bacillus species, Proteus species, Staphylococci etc. Sodium metabisulphite and ferric ammonium citrate are indicators of sulphate reduction used by C. perfringens, which produces black colonies. This medium is prepared by replacing animal peptones with vegetable defined peptones to avoid BSE/TSE/GMO risk.