Description

This medium is generally used for molecular and genetic studies, because of its nutritive capacity and simple composition. It is used for the cultivation and maintenance of recombinant strains of E. coli , originally derived from Escherichia coli strain K12, deficient in B vitamin production. These stains are specifically mutated to create an auxotrophic strain, unable to grow on a nutritionally deficient medium. This broth is a nutritionally rich medium due to the presence of casein enzymic hydrolysate and yeast extract. This allows the recombinant strains of E. coli to grow more rapidly since all the nutrients and essential growth nutrients required by these strains are readily available to them and they dont need to synthesize it themselves including B-vitamin.